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Proteintech wnt5a b
Wnt5a B, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wnt5a+b/WNT5A%2FB+Antibody/pm41906249-54-8-37
Average 96 stars, based on 117 article reviews
wnt5a b - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Chromatin Immunoprecipitation:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Flow Cytometry:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Magnetic Resonance Imaging:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Biomarker Discovery:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Immunohistochemistry:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Western Blot:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Article Title: Shenling Tongqiao powder targets TGF-β1/Smad-Wnt/β-catenin axis to suppress epithelial-mesenchymal transition in chronic rhinosinusitis.
Article Snippet: Rat IL-1β and IFN-γ ELISA kits were purchased from Abcam, and TGF-β1 ELISA kits were purchased from Proteintech. .. Protein of rat naso-sinus mucosa tissues and cells were extracted by RIPA according to the general operation procedure of western blot, and TGF-β1 (1:2000, Proteintech, China), Smad3 (1:500, Proteintech, China), p-Smad3 (1:2000, Affinity, USA), Wnt5A/B (1:2000, Proteintech, China), β-catenin (1:10,000, Proteintech, China), E-cadherin (1:50,000, Proteintech, China), N-cadherin (1:10,000, Proteintech, China), α-SMA (1:2000, Proteintech, China), Vimentin (1:500, Proteintec, China), β-actin (1:2000, Proteintech, China) expression were analyzed. ..

Expressing:

Article Title: Shenling Tongqiao powder targets TGF-β1/Smad-Wnt/β-catenin axis to suppress epithelial-mesenchymal transition in chronic rhinosinusitis.
Article Snippet: Rat IL-1β and IFN-γ ELISA kits were purchased from Abcam, and TGF-β1 ELISA kits were purchased from Proteintech. .. Protein of rat naso-sinus mucosa tissues and cells were extracted by RIPA according to the general operation procedure of western blot, and TGF-β1 (1:2000, Proteintech, China), Smad3 (1:500, Proteintech, China), p-Smad3 (1:2000, Affinity, USA), Wnt5A/B (1:2000, Proteintech, China), β-catenin (1:10,000, Proteintech, China), E-cadherin (1:50,000, Proteintech, China), N-cadherin (1:10,000, Proteintech, China), α-SMA (1:2000, Proteintech, China), Vimentin (1:500, Proteintec, China), β-actin (1:2000, Proteintech, China) expression were analyzed. ..

other:

Article Title: CRB2 Facilitates Epithelial Ovarian Cancer Progression by Inducing Polarity Changes via Activation of the Wnt/PCP Signalling Pathway.
Article Snippet: The antibodies used include: CRB2 (ab175546, Abcam, USA), Wnt5a/b (55184–1- AP), VANGL2 (21492–1- AP), JUN (24909–1- AP), JNK (24164–1- AP), RAC1 (24072–1- AP), β- TUBULIN (80713–1- RR), ATP1B1 (85916–1- RR), and GM130 (11308–1- AP), all purchased from Proteintech (Wuhan, China), FZD6 (DF4930) and DVL2 (DF4454), purchased from Affinity Biosciences (USA), and Cy3 Goat Anti- Rabbit IgG (H + L) Antibody (K1209), obtained from APExBIO (USA).

Blocking Assay:

Article Title: PCSK9 inhibitor alleviates experimental pulmonary fibrosis-induced pulmonary hypertension via attenuating epithelial-mesenchymal transition by suppressing Wnt/β-catenin signaling in vivo and in vitro
Article Snippet: .. After blocking, membranes were incubated with primary antibodies against β-actin (1:7000), E-cadherin (1:20000), N-cadherin (1:7000), Vimentin (1:20000), Snail (1:700), Wnt5A/B (1:2000), and β-catenin (1:10000) (Proteintech, China), followed by secondary antibody incubation (1:10000, Proteintech). .. Protein bands were visualized using ECL detection reagents and quantified with ImageJ software (V1.8.0.112).

Incubation:

Article Title: PCSK9 inhibitor alleviates experimental pulmonary fibrosis-induced pulmonary hypertension via attenuating epithelial-mesenchymal transition by suppressing Wnt/β-catenin signaling in vivo and in vitro
Article Snippet: .. After blocking, membranes were incubated with primary antibodies against β-actin (1:7000), E-cadherin (1:20000), N-cadherin (1:7000), Vimentin (1:20000), Snail (1:700), Wnt5A/B (1:2000), and β-catenin (1:10000) (Proteintech, China), followed by secondary antibody incubation (1:10000, Proteintech). .. Protein bands were visualized using ECL detection reagents and quantified with ImageJ software (V1.8.0.112).



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Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, <t>Wnt5a,</t> and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
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Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, <t>Wnt5a,</t> and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
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Image Search Results


Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, Wnt5a, and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.

Journal: Frontiers in Immunology

Article Title: PD0325901 alleviates thrombin-inhibited osteogenic differentiation through an IL-1β-activated feedback loop between MEK-Erk1/2 and NF-κB signal pathways: insights from bioinformatics and experimental verification

doi: 10.3389/fimmu.2026.1730337

Figure Lengend Snippet: Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, Wnt5a, and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.

Article Snippet: The following antibodies were used in this study: β-actin (AF5001, Beyotime, China), Col1α1 (ab270993, Abcam, UK), Runx2 (AF2593, Beyotime, China), OCN (AF6297, Beyotime, China), Igf1 (AF7179, Beyotime, China), Wnt5a (29793-1-AP, Proteintech, USA), Tgfb3 (AF8142, Beyotime, China), Spp1 (A5427, Bimake, USA), COX-2 (F0327, Bimake, USA), Matrix Metalloproteinase-9 (MMP-9, AF5234, Beyotime, China), Minichromosome Maintenance Complex Component 2 (MCM2, A5172, Bimake, USA), Proliferating Cell Nuclear Antigen (PCNA, SC-25280, Santa, USA), PAR-1 (AF6837, Beyotime, China), IL-1RA (AF7218, Beyotime, China), p65 (8242S, CST, USA), p-p65 (3033T, CST, USA), Erk1/2 (4695T, CST, USA), p-Erk1/2 (4370S, CST, USA), p-Stat3 (Y705) (9145S, CST, USA), p-Stat3 (S727) (9134S, CST, USA), and Stat3 (4904T, CST, USA).

Techniques: Biomarker Discovery, Derivative Assay, Western Blot